Uridine and Guanosine Nucleotides of Hen Oviduct
نویسندگان
چکیده
منابع مشابه
Structure and distribution of a disaccharide-carrying nucleotide and related nucleotides in hen oviduct.
A comparison has been made among the free nucleotide patterns of three different regions (magnum, isthmus, and uterus) of hen oviduct. More than 30 nucleotides have been separated from the three regions and characterized. One of the nucleotides isolated from the isthmus region has been shown, by a series of degradative procedures followed by comparison with authentic samples and oxidation with ...
متن کاملIsolation of Uridine Diphosphate N-Acetylglucosamine-6- phospho-1-galactose from Hen Oviduct
The isolation and identification of uridine diphosphate N-acetylglucosamine 6-phosphate from extracts of hen oviduct was first reported by Strominger in 1955 (1). Since that time, essentially no information concerning the metabolic role of this compound has become available. Recently, however, brief reports from two independent laboratories (2, 3) have described the isolation of a modified comp...
متن کاملThe enzymatic desulfation of sulfated sugar nucleotides by a novel sulfatase in hen oviduct.
A soluble fraction from the isthmus region of hen oviduct catalyzes the liberation of SOF from UDP-N-acetylgalactosamine-4-sulfate (the nucleotide that accumulates in the same tissue during egg formation). The enzyme is purified some &fold and is shown to catalyze the reactions: (a) UDP-N-acetylgalactosamine-4-sulfate 4 UDP-N-acetylgalactosamine plus SOF; and (b) UDP-N-acetylgalactosamine-4,6-d...
متن کاملEnzymatic Sulfation of Mucopolysaccharides in Hen Oviduct
An enzymatic reaction has been identified in the isthmus of hen oviduct in which sulfate is transferred from 3’-phosphoadenosine 5’-phosphosulfate to mucopolysaccharide acceptors (1). From studies of the reaction with monosaccharides and oligosaccharides as model acceptors (‘2) it was concluded that one or more enzymes in the preparations employed catalyze a direct transfer of sulfate from PAPS...
متن کاملThe polyA regions of hen oviduct RNA.
Total RNA from hen oviduct has been hydrolysed with a mixture of T(1) and pancreatic ribonucleases. Poly(A) tracts in the digestion product have been isolated by binding to oligo(dT) cellulose. Of the four major ribonucleotides, the product has been shown to contain only adenylic acid. When separated on polyacrylamide gels, the poly(A) gave two peaks corresponding to average apparent lengths of...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1962
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)83711-0